Journal: Human Cell

Article Title: β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression

doi: 10.1007/s13577-024-01081-y

Figure Lengend Snippet: β-Sitosterol inhibited hepatocellular carcinoma cell viability and proliferation in vitro. A β-Sitosterol chemical structure. B After treating Huh-7, HCCLM3, and 293 T cells with β-Sitosterol of gradient concentrations (5 μg/mL, 10 μg/mL, and 20 μg/mL) for 24, 48, and 72 h, respectively; Huh-7, HCCLM3, and 239 T cell viability was detected using MTT assay. C Following a 48 h treatment of Huh-7 and HCCLM3 cells with β-Sitosterol (5 μg/mL, 10 μg/mL, and 20 μg/mL), Huh-7 and HCCLM3 cell proliferation was detected using ( C ) colony formation assay and ( D ) EdU assay. Scale bar = 50 µm. All N = 3 (biologic replicates). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control group

Article Snippet: Huh-7 and HCCLM3 cells were incubated with 5 μg/mL, 10 μg/mL, or 20 μg/mL of β-Sitosterol for 24, 48 or 72 h. CHIR-98014 (MedChem Express, Shanghai, China) was treated to Huh-7 and HCCLM3 cells at 50 nM concentration for 48 h [ ].

Techniques: In Vitro, MTT Assay, Colony Assay, EdU Assay, Control

Journal: Human Cell

Article Title: β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression

doi: 10.1007/s13577-024-01081-y

Figure Lengend Snippet: β-Sitosterol enhanced apoptosis and affected the cell cycle of hepatocellular carcinoma cells. After a 48 h treatment with β-Sitosterol (5 μg/mL, 10 μg/mL, and 20 μg/mL), flow cytometry was used to detect the ( A ) cell cycle and ( B ) apoptosis of Huh-7 and HCCLM3 cells; ( C ) BAX, BCL2, cleaved caspase3, and total caspase3 protein levels within Huh-7 and HCCLM3 cell lines were detected using Western blot. All N = 3 (biologic replicates). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control group

Article Snippet: Huh-7 and HCCLM3 cells were incubated with 5 μg/mL, 10 μg/mL, or 20 μg/mL of β-Sitosterol for 24, 48 or 72 h. CHIR-98014 (MedChem Express, Shanghai, China) was treated to Huh-7 and HCCLM3 cells at 50 nM concentration for 48 h [ ].

Techniques: Flow Cytometry, Western Blot, Control

Journal: Human Cell

Article Title: β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression

doi: 10.1007/s13577-024-01081-y

Figure Lengend Snippet: β-Sitosterol suppressed hepatocellular carcinoma cell migration, invasion, and EMT. After a 48 h treatment of Huh-7 and HCCLM3 cell lines with β-Sitosterol (5 μg/mL, 10 μg/mL, and 20 μg/mL), Huh-7 and HCCLM3 cell migratory ability was detected using ( A ) the scratch assay; ( B ) Huh-7 and HCCLM3 cell invasive ability was detected using Transwell assay; ( C ) E-cadherin, N-cadherin, Snail, and Vimentin protein levels within Huh-7 and HCCLM3 cell lines were detected using Western blot. Scale bar = 100 µm. All N = 3 (biologic replicates). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control group

Article Snippet: Huh-7 and HCCLM3 cells were incubated with 5 μg/mL, 10 μg/mL, or 20 μg/mL of β-Sitosterol for 24, 48 or 72 h. CHIR-98014 (MedChem Express, Shanghai, China) was treated to Huh-7 and HCCLM3 cells at 50 nM concentration for 48 h [ ].

Techniques: Migration, Wound Healing Assay, Transwell Assay, Western Blot, Control

Journal: Human Cell

Article Title: β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression

doi: 10.1007/s13577-024-01081-y

Figure Lengend Snippet: The target gene of β-Sitosterol was analyzed through network pharmacology. A GSK3B expression in dataset GSE112790 was identified by t test analysis. *** p < 0.001, vs. the control group. B GSK3B expression in the TCGA-LIHC database was identified. C Kaplan–Meier analysis was conducted to analyze the correlation between GSK3B and hepatocellular carcinoma patients’ prognosis and survival. D the accuracy of GSK3B in prognostic risk diagnosis for hepatocellular carcinoma was analyzed using the ROC curve

Article Snippet: Huh-7 and HCCLM3 cells were incubated with 5 μg/mL, 10 μg/mL, or 20 μg/mL of β-Sitosterol for 24, 48 or 72 h. CHIR-98014 (MedChem Express, Shanghai, China) was treated to Huh-7 and HCCLM3 cells at 50 nM concentration for 48 h [ ].

Techniques: Expressing, Control, Biomarker Discovery

Journal: Human Cell

Article Title: β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression

doi: 10.1007/s13577-024-01081-y

Figure Lengend Snippet: β-Sitosterol suppressed hepatocellular carcinoma cell proliferation and migration and enhanced the apoptosis via inhibiting GSK3B expression. After a 48 h transfection of Huh-7 and HCCLM3 cells with GSK3B overexpression vector, and then a 48 h treatment (24, 48 and 72 h treatment for CCK-8) with β-Sitosterol (10 μg/mL), ( A ) GSK3B-protein level within Huh-7 and HCCLM3 cell lines was detected using Western blot; ( B ) cell viability was detected using CCK-8 assay; ( C ) The proliferation of Huh-7 and HCCLM3 cells was detected using colony formation assay; the Huh-7 and HCCLM3 cell ( D ) cycle and ( E ) apoptosis were detected using flow cytometry; ( F ) Huh-7 and HCCLM3 cell invasion was detected using Transwell assay. Scale bar = 100 µm. All N = 3 (biologic replicates). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vector-NC group; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the β-Sitosterol group

Article Snippet: Huh-7 and HCCLM3 cells were incubated with 5 μg/mL, 10 μg/mL, or 20 μg/mL of β-Sitosterol for 24, 48 or 72 h. CHIR-98014 (MedChem Express, Shanghai, China) was treated to Huh-7 and HCCLM3 cells at 50 nM concentration for 48 h [ ].

Techniques: Migration, Expressing, Transfection, Over Expression, Plasmid Preparation, CCK-8 Assay, Western Blot, Colony Assay, Flow Cytometry, Transwell Assay

Journal: Human Cell

Article Title: β-Sitosterol alleviates the malignant phenotype of hepatocellular carcinoma cells via inhibiting GSK3B expression

doi: 10.1007/s13577-024-01081-y

Figure Lengend Snippet: Comparison of the effects of β-Sitosterol and GSK-3 inhibitor on hepatocellular carcinoma cells. Huh-7, and HCCLM3 cells were treated with β-Sitosterol (10 μg/mL) and/or GSK3B inhibitor (50 nM CHIR-98014) for 48 h, and then examined for: ( A ) GSK3B-protein level by Western blot; ( B ) cell viability using CCK-8 assay; ( C ) cell proliferation using colony formation assay; ( D – E ) cell cycle and apoptosis using flow cytometry; ( F ) cell invasion using Transwell assay. Scale bar = 100 µm. All N = 3 (biologic replicates). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the CHIR-98014 + β-Sitosterol group. & p < 0.05, && p < 0.01, &&& p < 0.001 compared to the CHIR-98014 group

Article Snippet: Huh-7 and HCCLM3 cells were incubated with 5 μg/mL, 10 μg/mL, or 20 μg/mL of β-Sitosterol for 24, 48 or 72 h. CHIR-98014 (MedChem Express, Shanghai, China) was treated to Huh-7 and HCCLM3 cells at 50 nM concentration for 48 h [ ].

Techniques: Comparison, Western Blot, CCK-8 Assay, Colony Assay, Flow Cytometry, Transwell Assay, Control